Coding

Part:BBa_K3782005:Design

Designed by: Jana Näf   Group: iGEM21_UNILausanne   (2021-10-17)


Rhagium inquisitor ice-binding protein gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 130
    Illegal AgeI site found at 163
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was codon optimized for optimal expression in Escherichia coli.


Source

The source of RiAFP is the Siberian cerambycid beetle Rhagium inquisitor. Its sequence was retrieved from GenBank. https://www.ncbi.nlm.nih.gov/nuccore/HQ540314.1

References